Determination of effective synaptic conductances using somatic voltage clamp
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چکیده
منابع مشابه
Determination of Effective Synaptic Conductances Using Somatic Voltage Clamp
The interplay between excitatory and inhibitory neurons imparts rich functions of the brain. To understand the underlying synaptic mechanisms, a fundamental approach is to study the dynamics of excitatory and inhibitory conductances of each neuron. The traditional method of determining conductance employs the synaptic current-voltage (I-V) relation obtained via voltage clamp. Using theoretical ...
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The voltage clamp technique is frequently used to examine the strength and composition of synaptic input to neurons. Even accounting for imperfect voltage control of the entire cell membrane ("space clamp"), it is often assumed that currents measured at the soma are a proportional indicator of the postsynaptic conductance. Here, using NEURON simulation software to model somatic recordings from ...
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The interaction between excitation and inhibition is crucial for brain computation. To understand synaptic mechanisms underlying brain function, it is important to separate excitatory and inhibitory inputs to a target neuron. In the traditional method, after applying somatic current or voltage clamp, the excitatory and inhibitory conductances are determined from the synaptic current-voltage (I-...
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The voltage-clamp technique is applicable only to spherical cells. In nonspherical cells, such as neurons, the membrane potential is not clamped distal to the voltage-clamp electrode. This means that the current recorded by the voltage-clamp electrode is the sum of the local current and of axial currents from locations experiencing different membrane potentials. Furthermore, voltage-gated curre...
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This course module introduces the analysis of data from intracellular patch-clamp recordings in the voltage clamp mode, focusing on the detection and statistical description of spontaneous as well as evoked post-synaptic currents (PSCs). After having completed the module, students should be able to detect PSCs by setting appropriate thresholds and analyze amplitude distribution, reliability and...
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ژورنال
عنوان ژورنال: PLOS Computational Biology
سال: 2019
ISSN: 1553-7358
DOI: 10.1371/journal.pcbi.1006871